N-PEP-12 counteracts age-related neurodegeneration

N-PEP-12 improves cognitive function in elderly

A decline of cognitive performance in association with aging is a widespread condition in elderly humans. Many well-controlled clinical studies* have demonstrated that learning and memory abilities, important in daily life, decline markedly in the course of normal aging. This common phenomenon has been termed Age-Associated Memory Impairment (AAMI)

N-PEP-12 was chosen as a candidate to study in AAMI because of its excellent safety profile, mechanism of action and preliminary evidence of efficacy in both animals and humans.

In a study with elderly volunteers who met criteria of AAMI, the activating effects of N-PEP-12 could be confirmed. Assessments of cognitive performance were done at baseline (before the intake of N-PEP-12) and at the end of the test period (after one month) using a self-assessment questionnaire.

Increased responsiveness after 1 month of treatment

After one month intake of N-PEP-12 significant improvement was claimed by participants:

  • 90% improved in at least one of the domains
  • 70% had improved memory function
  • 45% reported better concentration
  • 30% noted that their learning ability increased significantly and
  • 60% reported a generally better well-being.

Further the study confirmed that N-PEP-12 treatment is absolutely safe and well tolerated and that it constitutes a promising compound for treating memory deficits that occur so frequently in later life as to be considered "normal".

Cognitive Effects of the Novel Neuroprotective Dietary Supplement N-PEP-12: Evidence from a Self-Assessment Study

D. Volc et al./Jatros Neurologie & Psychiatrie 2005
First-last names and the grocery list selective reminding test: Two computerized measures of everyday verbal learning

R.Youngjohn et al./Clinical Neuropsyc. 1991, 6(4):287-300
Assessment of Memory Complaint in Age-Associated Memory Impairment: The MAC-Q
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Changes in facial recognition memory across the adult life span
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N-PEP-12 improves recognition memory and neuronal plasticity

Aging is a stage of life where cognitive and motor functions are impaired. This is because Oxidative and inflammatory processes worse neurodegeneration, which affects dendritic morphology and neuronal communication of limbic regions with memory loss. The study from Hernández-Hernández in 2018 evaluates the effect of N-PEP-12 on recognition memory and motor activity, as well as its effects on dendritic morphology and the immunoreactivity of Synaptophysin (SYP), Brain Derived Neurotrophic Factors (BDNF) and other factors in the several brain regions.

The recognition memory of the groups treated with N-PEP-12 and vehicle (water drinking) was determined by the Discrimination Index (DI), which consisted of quantifying the latency time of each animal when exploring a novel object compared to the examining latency of a familiar object.

As the figure shows N-PEP-12 induces an improvement in recognition memory by mean of NORT (The novel object recognition test) in old rats.

       Effects of N-PEP-12 on recognition memory

The morphological analysis in this trial indicates that N-PEP-12 also increases the density of dendritic spines and the total dendritic length in neurons of the prefrontal cortex, when compared with the aged rats that only received the vehicle.

For Example for PFC-3

Measuring the effect of N-PEP-12 administration on the immunoreactivity of Synaptophysin and brain-derived neurotrophic factors shows that N-PEP-12 treatment promoted a process of plasticity in the cerebral regions of the limbic system in aging rats.

Therefore it can be observed, that the treatment with N-PEP-12 promotes neuronal plasticity, evidenced by the increase in the dendritic morphology of pyramidal neurons, which coexists with the highest immunoreactivity for SYP and BDNF.

These results of the trials suggest that treatment with N-PEP-12 induces modifications in neuronal and cognitive plasticity in the limbic system, which contributes to improving recognition memory during the aging process.

Data from the recognition memory have a strong evidence of the therapeutic potential of N-PEP-12 in aging rats by improving recognition memory compared with the vehicle-only group.

In this sense, N-PEP-12 can be considered as a therapeutic alternative to delay the behavioral, cellular, and molecular deteriorations that appear during aging process, and opens a new opportunity to improve the quality of life of elderly people, struggling with cognitive impairment.

The neuropeptide-12 improves recognition memory and neuronal plasticity of the limbic system in old rats

E.M. Hernández-Hernández et al./Synapse 2018, 72(8), e22036